Test ID:
706585
CPT code:
81261; 81264
Synonyms:
B-Cell Gene Rearrangements Profile, IgH and IgK
IgH Clonality Assay
IgH Gene Rearrangement Assay
IgK Clonality Assay
IgK Gene Rearrangement Assay
Clinical Use:
This profile can be used to detect clonal B-cell immunoglobulin heavy chain (IgH) and immunoglobulin κ light chain (IgK) gene rearrangements in blood, bone marrow, and tissue specimens with combined B-cell clonality detection rate of 99%. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a B-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive condition. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.
Specimen Type:
Whole blood, bone marrow, formalin-fixed, paraffin-embedded (FFPE) tissue block or slides, fresh or frozen tissue
Requested Volume:
3 to 5 mL whole blood, 1 mL bone marrow, five unstained slides at 10 μM or formalin-fixed, paraffin-embedded tissue block, tissue in RPMI or frozen
Minimum Volume:
0.5 mL whole blood, 0.5 mL bone marrow, two unstained slides at 10 μM
Container Type:
Lavender-top (EDTA) tube, yellow-top (ACD) tube, green-top (heparin) tube, FFPE tissue block or slides, or tissue in RPMI or frozen
Collection:
Fresh tissue should be placed in RPMI medium or shipped frozen on dry ice. Small samples (eg, punch biopsies, fine-needle aspirates) will also be accepted. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.
Storage Instructions:
Maintain blood, bone marrow or tissue in RPMI at 2°C to 8°C; FFPE specimens at room temperature; tissue at -80°C.
Rejection Criteria
Frozen whole blood/bone marrow; quantity not sufficient for analysis; tumor tissue; broken or stained slides